Processing another sample with TopHat and HTSeq¶

Rather typing all of this in - let’s put this in a text file, so we can run the TopHat and HTSeq commands all at once! This is called a “script”.

Type:

cd /mnt/work
cat > chick-tophat-2.sh <<EOF


and then paste this in:

# go to the working directory
cd /mnt/work

# now run Tophat!
tophat \
-G cuffmerge_all/nostrand.gtf \
--transcriptome-index=/mnt/genome/tophat/merged \
-o tophat_female_repl2 \
/mnt/genome/Gallus_gallus/UCSC/galGal3/Sequence/Bowtie2Index/genome \
female_repl2_R1.qc.fq.gz female_repl2_R2.qc.fq.gz

htseq-count --format=bam --stranded=no --order=pos \
tophat_female_repl2/accepted_hits.bam \
cuffmerge_all/nostrand.gtf > female_repl2_counts.txt

EOF


(Be sure to press the Enter or Return key after pasting this in!) This is called a ‘heredoc’ and it gives a way to write a shell script via copy-paste ;).

Next, type:

bash chick-tophat-2.sh


This will run all of the commands in the file ‘chick-tophat-2.sh’.

You can use the ‘nano’ editor to modify this file – type:

nano chick-tophat-2.sh


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